This work involved an exhaustive review and update to the underlying taxonomy database which was used in conjunction with NCBI's type strain Entrez filter to retrieve candidate sequences. The sequence data and their associated taxonomy/meta-data have been reviewed and corrected to include the most up-to-date information. If a sequence failed validation or could not be accurately validated, it was excluded. These reference sequences can now be used as ‘gold standards’ for the analysis of existing and new rRNA sequences.