Chunk #31 — MATERIALS — REAGENT SETUP — mTeSR1 culture medium

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Genome engineering using the CRISPR-Cas9 system.

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For culture of human embryonic stem cells (hESCs), prepare mTeSR1 medium by supplementing it with the supplement solution supplied with the medium and 100 μg ml–1 Normocin. Prepared medium can be stored at 4 °C for up to 2 months.