Chunk #30 — MATERIALS — REAGENT SETUP — D10 culture medium

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Genome engineering using the CRISPR-Cas9 system.

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For culture of HEK 293FT cells, prepare D10 medium by supplementing DMEM with GlutaMAX and 10% (vol/vol) FBS. For routine cell line culture and maintenance, D10 medium can be further supplemented with 1× penicillin-streptomycin. Store the medium at 4 °C for up to 1 month.