equal volume of the vehicle and provide access to two bottles, one with water and the other with increasing concentrations of ethanol over the course of the experiment. We found that CVC treatment did not change the overall consumption of ethanol per kilogram body weight (Fig. S1A, supplementary). CVC treatment also had no significant effect on alcohol preference (Fig. S1B, supplementary) compared to vehicle-treated controls (consumption interaction F(3, 56) = 0.6927; p = 0.5603 and treatment groups F(1, 56) = 1.341; p = 0.2518; preference interaction F(3, 56) = 0.9346; p = 0.4301 and treatment groups F(1, 56) = 1.465; p = 0.2312). The total fluid was also measured as grams of ethanol and water consumed and normalized to body weight and was not statistically different between treated and untreated mice (interaction F(3, 56) = 0.046; p = 0.9869 and treatment groups F(1, 56) = 3.176; p = 0.0801) (Fig. S1C, supplementary).
