The Lieber-DeCarli alcohol delivery model relies on alcohol dissolved in a liquid diet to ensure mice receive sufficient daily alcohol for study purposes. Because the alcohol is dissolved in the animals’ only source of calories, there is limited expected variation in alcohol consumption across the groups. Indeed, treatments with CVC did not significantly alter the daily liquid diet consumption compared with vehicle-treated controls. The average daily consumption of 5% ethanol in the Lieber-DeCarli liquid diet for vehicle-treated mice was 18.73 ± 4.0 mL compared with 6wk CVC-treated mice 19.37 ± 3.6 mL (p = 0.37) and 3wk CVC-treated mice 19.79 ± 3.7 mL (p = 0.13). Therefore, to test whether CVC treatment could have an impact on alcohol consumption or preference, we used a two-bottle choice test model. Mice were treated with daily i.p. injections of CVC or with an equal volume of the vehicle and provide access to two bottles, one with water and the other with increasing concentrations of ethanol over the course of the experiment. We found that CVC treatment did not change the overall consumption of
