Taken together, these results show that the induced Mecp2e1 (but not Mecp2e2) expression is associated with reduced DNA methylation at the Mecp2 REs and decreased global 5mC DNA methylation. Hence, our findings implicate the possible role of Mecp2 gene-specific DNA demethylation at the specific REs on the expression of Mecp2e1/MeCP2E1, and MeCP2 (total) at D2. Moreover, altered expression of Mecp2 isoforms without any change in DNA methylation at the Mecp2 REs at D8 imply that mechanisms other than DNA methylation could be involved in downregulating Mecp2 isoforms.
