In addition, the majority of RNA-seq data we analyzed lacks strand information and as a result most of the lincRNAs in our catalog are of ambiguous strandedness. Prior annotations have relied upon splice site orientation to infer the strandedness of the transcript [16]. While this is a reasonable approach that we too have adopted when applicable in the present lincRNA catalog, stranded RNA-seq data is needed to most confidently assign strandedness to de novo assembled transcripts.
