vitro transcription was done, followed by site-specific cleavage using MassCLEAVE biochemistry. MassARRAY compact MALDI-TOF mass was used to acquire spectral peaks and converted into methylation ratios using EpiTYPER software.66 Two statistical criteria were used to identify genes validated by sequenom; one with genes showing methylation difference of greater than 10% in at least one CpG probe; furthermore, another criteria was the methylation change in same direction of all CpG probes tested for that gene amplicon.
