GeneGo analysis of total DEGs and upregulated DEGs (S6 Table) revealed potential involvement of protein transport as well as the Wnt signaling pathway. Due to the small sample size together with the finding that <2 matched genes were observed for each GeneGo pathway, conclusions could not be drawn for the down-regulated DEGS using this particular analysis tool (data not shown). From Ingenuity Pathway Analysis (IPA), receptor signaling pathways were enriched between BPD and control L neurons (Table 3). Similar results were obtained when analysis was performed on the up-regulated DEGS only (Table 3). As for GeneGo, an analysis of down-regulated DEGs was not appropriate for IPA. KEGG pathway analysis on DEGs was also performed (S7 Table) and supported the results from the other analyses, identifying receptor-mediated signaling, RNA metabolism, and protein trafficking as major processes potentially dysregulated in BPD. Interestingly, many of the enriched pathways involved GSK3β, which has been implicated as a therapeutic target of lithium [42], the first-line treatment for BPD [43].
