Single cell suspensions of pMGLs were blocked with FcR blocking reagent, 1% BSA, to avoid non-specific antibody binding, in NGD medium without phenol red. 10^5 cells were labeled in 100μL with pre-conjugated rat anti-CD11B-FITC (1:100, StemCells Tech.), mouse anti-CD45-AF647 (1:100, Biolegend), and unconjugated rabbit anti-IBA1 (1:100, Abcam) followed by AF647 conjugated secondary. Sorting was performed on a customized FACS Aria (BD).
