For qualitative assessment of phagocytosis, 1μm polystyrene red-orange fluospheres (Thermo) were left to settle at a density of 107/cm2 on PEI coated plastic. pMGLs were added the next day at a density of 1000/cm2. Cells were observed after 6 hours, displaying their migration on the lawn and the cytoplasmic bead uptake. Alternatively, beads were added directly to a pMGL culture medium at a concentration of 108 beads/mL. Phagocytosis was observed in real time, highlighting the rapid active uptake.
