Animals were anesthetized with isoflurane and euthanized by decapitation. Coronal brain slices (400μm) were prepared as described previously (Lack et al., 2007) using modified aCSF (in mM: 180 Sucrose, 30 NaCl, 4.5 KCl, 1 MgCl2•6H20, 26 NaHCO3, 1.2 NaH2PO4, 10 glucose) containing 100μM ketamine. Slices from all experimental groups were transferred and stored in 0.5 liter of standard oxygenated aCSF solution (in mM: 126 NaCl, 3 KCl, 1.25 NaH2PO4, 2 MgSO4, 26 NaHCO3, 10 glucose, and 2 CaCl2) at room temperature for at least 1 hour and up to 6 hours.
