Ethanol exposure was accomplished via an ethanol vapor chamber similar to that used in other studies (Lack et al., 2007). Briefly, animals in their home cages were placed into air-tight, Plexiglas enclosures and exposed to either ethanol vapor (~28mg/L) or room air during the light cycle (12 hours/day) for 10 consecutive days. Animals receiving the chronic intermittent treatment were divided into those euthanized while still intoxicated at the end of the last exposure (CIE) and those euthanized 24 hours after the last exposure (WD). Tail blood was taken periodically during the exposure to monitor blood-ethanol concentration and trunk blood was collected on the day of sacrifice for subjects in the CIE group. Blood ethanol levels at the time the CIE animals were sacrificed were 225±32mg/deciliter.
