Chunk #10 — Materials and methods — DNA sequencing

Source

Deep resequencing of 17 glutamate system genes identifies rare variants in DISC1 and GRIN2B affecting risk of opioid dependence.

Embedded

yes

Text

The 76 DNA pools were barcoded, and paired-end sequenced with read-length 2×100 bp in seven lanes of an Illumina HiSeq analyzer at the Yale Center for Genome Analysis. Each of the first six lanes contained 11 barcoded DNA pools, and the seventh lane contained 10 bar coded DNA pools. Every lane included both case and control pools from AAs and EAs.