The target regions are the coding exons and their flanking intronic regions (at least 50bp from the exon) of the 17 genes. A total of 173 PCRs with a cumulative length of 75,152 bp were performed to cover the targeted regions of 34,544 bp. PCR conditions of all the primers were optimized individually. Then, the 173 PCR products from each of the 380 pooled DNA samples were combined. Five pools with the same sex, population group, and phenotypes of AD, CD and OD were pooled. In the end, 76 DNA pools with 20 individuals per pool were prepared for sequencing, including 16 AA case pools, 19 AA control pools, 22 EA case pools, and 19 EA control pools.
