We adopted a DNA pooling strategy to reduce the cost and maximize the utility of the high-throughput DNA sequencing technology. This strategy has been proven to be very successful in a number of previous studies (Bevilacqua et al., 2010; Rivas et al., 2011). First, four DNA samples with the same concentrations and volumes were pooled. The four pooled DNA samples were from subjects with the same sex, population, and phenotypes of AD, CD and OD. This resulted in 380 pooled DNA samples for PCR enrichment of the targeted genomic regions.
