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Chunk #7 — Materials and methods — DNA sequencing

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Deep resequencing of 17 glutamate system genes identifies rare variants in DISC1 and GRIN2B affecting risk of opioid dependence.
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DNA was extracted from blood, saliva, or cell lines. A detailed description regarding DNA quality and quantity is provided in the supplementary material. A total of 1520 DNA samples were selected for sequencing based on the subjects’ phenotypes (Table 1) and the DNA quality and quantity.