Fibroblasts (passages 3–8) were reprogrammed using CytoTune®-iPS kit (Life Technologies) following methods described previously [27]. Three iPSC lines with characteristic morphology were generated for each patient fibroblast cell line. Cytogenetic analysis (Cell Line Genetics) on 20 G-banded metaphase cells was performed from each cell line and all 20 cells demonstrated normal karyotype. No abnormal cells were detected. We randomly chose one clone from each patient for subsequent studies.
