Chunk #9 — Methods — Patient Population — Generation of human iPSCs

Source

Transcriptomic Analysis of Induced Pluripotent Stem Cells Derived from Patients with Bipolar Disorder from an Old Order Amish Pedigree.

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Selected iPSCs were expanded and cultured on irradiated mouse embryonic fibroblasts (MEF) (GlobalStem) in ES media, which contains DMEM/F12 (1:1) (HyClone) with 20% Knockout serum replacement (Invitrogen), 1X penicillin/Streptomycin (10U/ml/10μg/ml, Hyclone), 1X GlutaMax (Invitrogen), 1X Non-essential amino acids (Hyclone), 55 μM 2-mercaptoethanol (Gibco) and 10ng/ml human bFGF (R&D Systems) with daily media replacement.