Before initial differentiation, iPSCs were transferred to plates coated with Matrigel™ (BD Bioscience) following the manufacturer’s instruction and maintained in mTeSR1 media (StemCell Technologies) until MEF feeder cells were eliminated. Neural Induction Media (NIM) was prepared to contain DMEM/F12 (1:1) with 1X N2 supplements (Invitrogen), 1X penicillin/Streptomycin (10U/ml/10μg/ml, Hyclone), 1X GlutaMax (Invitrogen), 1X Non-essential amino acids (Hyclone). Neural Differentiation Media (NDM) was prepared by adding 1X N2 supplements, 1X B27 without vitamin A (Invitrogen), 1X penicillin/Streptomycin, 1X GlutaMax, 1X Non-essential amino acids (Hyclone) to Neurobasal media (Life Technologies).
