Chunk #10 — Materials and Methods — Preparation of nuclear and cytoplasmic extracts and electrophoretic gel mobility shift assay

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The opposite effects of acute and chronic alcohol on lipopolysaccharide-induced inflammation are linked to IRAK-M in human monocytes.

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A consensus double-stranded NFκB oligonucleotide (5′AGTTGAG GGGACTTTCGC3′) was used and EMSA was performed (19).