Chunk #9 — Materials and Methods — Preparation of nuclear and cytoplasmic extracts and electrophoretic gel mobility shift assay

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The opposite effects of acute and chronic alcohol on lipopolysaccharide-induced inflammation are linked to IRAK-M in human monocytes.

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Nuclear and cytoplasmic extracts from cells with or without stimulation at 37°C were performed by the method of Schatzle et al. (18) as described earlier (19).