In this study, we evaluated glucose homeostasis during HFD feeding in a few colonies of mice. The P+/A- and P-A- mice are new colonies created in our lab; HFD can deteriorate glucose intolerance in P+A- mice but not in P-A- mice. For observing an effect of HFD on glucose intolerance, the optimal fasting time was 6 h because the longer fasting times ( 18 or 24 h) diminished the HFD-enhancing effect on glucose intolerance (ref 46). In this study, the mice were fasted for 15 h before glucose tolerance test, and the HFD-enhancing effect on glucose intolerance was still observed in P+A- mice but not in P-A- mice. If a 6 h of fasting make the P-A- mice exhibit a HFD-enhancing effect, probably this HFD-enhancing effect in P-A- mice is still less than in P-A+ mice. The P-A+ mice are equivalent to ppara−/−mice and our observation with glucose homeostasis in P-A+ mice is consistent with others’ reports (ref. 14, 15). The cyp2a5−/− mice exhibited obvious glucose intolerance, which is consistent with our previous report that alcohol can induce hyperglycemia in
