The B6, D2, and 26 BXD strains of mice (8–10 mice per strain including both males and females) were divided into two groups: 1) saline group, treated with iusovolumetric saline via IP injection, or 2) ethanol group, treated with an IP injection of 1.8 g/kg i.p. ethanol (12.5% v/v). These conditions allowed us to test for differences in gene expression both in the presence and absence of alcohol. Four hours after treatment, these mice were sacrificed for tissue harvest.
