The 67 untreated BXD RI strains used to generate hippocampal expression data and the ethanol or saline treated animals (26 BXD RI strains) described above, were killed and harvested for their tissue according to previous methods [26]. Briefly, mice were anesthetized using Avertin (1.25% 2,2,2- tribromoethanol and 0.8% tert-pentyl alcohol in water; 0.8–1.0 ml, i.p.) and killed via cervical dislocation at 2–6 months of age. The brain was extracted and dissected to obtain the hippocampus. The cortex above the hippocampus and dentate gyrus was removed along the septotemporal axis. The exposed hippocampus and dentate gyrus were then taken out of the hemisphere in a ventral-to-dorsal direction. The left and right hippocampi were pooled and stored in RNAlater overnight at 4°C, then kept at 80°C until RNA extraction.
