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Chunk #12 — Materials and methods — RNA extraction and microarrays

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The effect of alcohol on the differential expression of cluster of differentiation 14 gene, associated pathways, and genetic network.
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RNA was extracted from the hippocampus using RNA STAT-60 (protocols can be found at Tel-Test, www.tel-test.com) as per the manufacturer’s instructions. A spectrophotometer (Nanodrop Technologies, found at http://www.nanodrop.com) was used to measure RNA concentration and purity, and the Agilent 2100 Bioanalyzer was used to evaluate RNA integrity. The RNA integrity values had to be greater than 8 to pass quality control. The majority of samples had values between 8 and 10.