paperKB
coga / coga-kb
Help
Sign in

Chunk #13 — Materials and methods — RNA extraction and microarrays

Source
The effect of alcohol on the differential expression of cluster of differentiation 14 gene, associated pathways, and genetic network.
Embedded
yes

Text

The gene expression data of the first set of 67 BXD RI strains used for system genetics analysis were collected using the Affymetrix Mouse Genome M430 2.0 array based on the manufacturer’s protocol. The gene expression data of the second set of 26 BXD RI strains under saline and ethanol treatment used for analysis of the effect of ethanol on gene expression were collected using the Illumina Sentrix Mouse-6 v1.1 arrays based on the manufacturer’s protocol. Both the first (“Hippocampus Consortium M430v2”) and second data sets (“UTHSC BXD Hippocampus ILM v6.1 NOS Balanced (Feb17) RankInv” and “UTHSC BXD Hippocampus ILM v6.1 NOE Balanced (Feb17) RankInv”) are listed in our GeneNetwork website (www.genenetwork.org). Detailed information for these data sets, including strain, age, sex, experimental protocol, data quality control, etc. can be found in the “info” pages.