We generated iPSCs from peripheral blood mononuclear cells isolated from 91 individuals, predominantly African Americans (43%) and European Americans (55%), with more women (60%) than men (40%) (Table S1). All established iPSC lines were confirmed to be free of exogenous Sendai viral reprogramming factor expression and then tested for pluripotency by fluorescence-activated cell sorting (FACS) staining for SSEA4 and Tra-1-60 (Figure S1A, Table S1). Samples passing these criteria were differentiated into HLCs. Differentiated HLCs were morphologically similar to primary hepatocytes, and for a subset of HLC samples, expression of HNF4α was confirmed by immunofluorescence (Figure S1B). The HLCs also secreted apolipoprotein B (apoB), triglycerides, and albumin (Figure S2A), a set of properties unique to functional hepatocytes.
