Mean (±SD) GAD67 mRNA levels were significantly 37.2% lower (t12=7.11; p<0.001) in GAD67+/− mice (664.9 ± 39.0 nCi/g) relative to WT mice (1059.4 ± 141.4 nCi/g) (Figure 1A–C). Mean CB1R mRNA levels were also significantly 15.7% lower (t12=2.35; p=0.036) in GAD67+/− mice (402.1 ± 56.8 nCi/g) relative to WT mice (477.0 ± 62.4 nCi/g) (Figure 1D–F). Furthermore, GAD67 and CB1R mRNA levels were positively correlated across all mice (r=0.61; p=0.010; Figure 2). In contrast, DAGLα mRNA levels did not differ (t12=0.0; p=0.999) between groups (Figure 1G–I).
