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Chunk #42 — Materials and methods — Fear conditioning

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Alcohol drinking exacerbates neural and behavioral pathology in the 3xTg-AD mouse model of Alzheimer's disease.
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modified to conceal contextual cues by using an acrylic insert to change wall and floor surfaces; additionally, a mild novel odor (dilute vanilla flavoring) was added to the sound attenuating box. Mice were then placed into the modified chamber and allowed to explore for 2min, then the acoustic stimulus was presented for the remaining 3min of the session. Freezing behavior before and during the stimulus were recorded.