pMGLs cytokine profiles in response to endotoxin challenge(a) Baseline cytokine profiler assay from 105 pMGLs conditioning 2mL of NGD in 24 hours. Top panel: 10′ exposure, showing baseline secretion of detectable CCL2, MIP1α/β, CXCL1 and IL8. Lower Panel: profile after stimulation for 24hours with 100ng/mL LPS and 20ng/mL IFN-γ. IL6, TNFα, MIP1α/β and CXCL10 display a dramatic increase. (b) map, to scale, of the spots blotted in panel a. Green highlights cytokines showing a significant upregulation. (c, d) Quantitative qPCR of TNFα (c) and IL6 (d) transcription in pMGLs at baseline (−) and after LPS stimulation (+). Data are presented as mean ± s.e.m. from 2 biological replicates, t-test significance is reported.
