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Chunk #8 — Materials and Methods — Genotyping

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ALDH2 is associated to alcohol dependence and is the major genetic determinant of "daily maximum drinks" in a GWAS study of an isolated rural Chinese sample.
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116 pedigrees ranging in size from 2 to 27 individuals with an additional 100 individuals unrelated to anyone else in the sample. Mendelian errors, detected on the basis of unshared alleles with parents and likely resulting from miscalls in the genotyping, were detected using SimWalk2 (Sobel & Lange 1996, Sobel et al. 2001, Sobel et al. 2002). Across all loci, an average of 39.4 genotypes were identified and changed to no calls so that no Mendelian errors remained in the pedigree. This represents an approximate miscall rate of 0.013%.