C567BL/6 and C57BL/6 Ins2 WT/C96Y were obtained from Jackson Laboratories. Txnip−/− mice were generated as previously described (Hui et al., 2008). Txnip−/−; Ins2 WT/C96Y mice were generated by breeding Txnip−/− and Ins2 WT/C96Y mice and are both on the C57BL/6 background. All procedures described involving animals were performed in accordance with protocols approved by the Institutional Animal Care and Use Committee at the University of California, San Francisco. Animals were maintained in a specific pathogen-free animal facility on a 12-h light–dark cycle at an ambient temperature of 21 °C. They were given free access to water and food. All experiments used age-matched male mice.
