1-stearoyl-2-arachidonoylglycerol (SAG), 2-AG, AA, and their deuterated analogs were purchased from Cayman Chemicals (Ann Arbor, MI). N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and 4-(dimethylamino)pyridine (DMAP) were purchased from Aldrich Chemical Company (Milwaukee, WI) and used without further purification. Solvents were HPLC grade and purchased from Fisher Scientific (Pittsburg, PA). 1-oleoyl-2-arachidonoylglycerol (OAG) was synthesized by a reaction of 2-AG and oleic acid using EDC and DMAP (catalyst) in heated toluene (60°C). The reaction was quenched with water. The organic compounds were extracted with ethyl acetate and the product was purified with preparative HPLC.
