Chunk #24 — Results and Discussion — Gene expression differences between isolation methods are associated with cellular composition and B-cell manipulation
In the LCLs, 1,300 and 2,329 probes were uniquely expressed as compared to B-cell CD19 and CD20 (Figure 5). These probes showed an enrichment of the GO term "Cell cycle phase" (GO:00022403, FDR P = 0.005 and FDR P = 1.5*10-4) and included genes involved in mitosis (CCNB1, CENPF, PBK, TTK). Between the B-cell CD19 or B-cell CD20 and LCL samples, 496 (4.7%) and 472 (4.9%) probes were differentially expressed. In both B-cell CD19 and CD20 samples, glycolysis GO terms were enriched whereas cell cycle GO terms were only enriched in B-cell CD19 (Table 4). The GO analysis suggested an increased rate of glycolysis and cell cycle in LCLs as compared with B-cells. B lymphocytes found in the peripheral circulation are in a non-proliferative state and require stimulation with an antigen to enter the cell cycle. Hollyoake et al. showed that infection with EBV causes the infected cells to activate the cell-division cycle [29].
