Chunk #23 — Results and Discussion — Gene expression differences between isolation methods are associated with cellular composition and B-cell manipulation
To prevent the difficulties of cell type mixtures, B-cell specific methods have been developed. To investigate to which extent B-cell specific methods differ from each other, we compared gene expression measurements of LCLs with B-cell CD19 and B-cell CD20. For the B-cell CD19 and CD20, 1,557 and 1,136 probes were uniquely expressed compared with the LCLs (Figure 5). In both B-cell CD19 and CD20 the GO term (GO:0009611) "response to wounding" (FDR P = 3.8*10-9 and FDR P = 1.5*10-11) was most significantly enriched. This category contained B-cell specific genes encoding complement pathway components (CD40lg, CD180), interleukins (IL-6), chemokine receptors (CCR2, CCR3), immunoglobulin receptors (FCER1G) and members of the toll-like receptor family (TLR4, TLR8) (see Additional file 3).
