Deglycosylation with PNGase F of the wild-type and A118G mutant of hMOPR (A118/N40-hMOPR and G118/N40-hMOPR, respectively) stably expressed in CHO cellsCells were solubilized with 2% Triton X-100. The A118/N40- or G118/D40-hMOPR was partially purified by WGL affinity chromatography, left untreated or treated with PNGase F and resolved with 8% SDS–PAGE as described in the Fig. 3 legend, and immunoblotted with HA.11 described in the Fig.4 legend. The amount of eluate was adjusted to have similar MOPR levels. Each figure shown is from one of three independent experiments with similar results.
