Immunoblotting of the wild-type and A118G mutant of hMOPR (A118/N40-hMOPR and G118/D40-hMOPR, respectively) stably expressed in CHO and HEK293 cellsCHO cells were stably transfected with 3HA tagged A118/N40- or G118/D40-hMOPR cDNA in the mammalian expression vector pcDNA3. Cells from stable mixed clones were collected and the protein contents resolved with 8% SDS–PAGE and immunoblotted with monoclonal anti-HA antibody HA.11. After stripping, the same blot was then processed for immunoblotting with mouse anti-GAPDH-HRP-conjugated. Each figure is from one of two independent experiments performed with similar results.
