Deglycosylation with PNGase F of MOPRs from thalami of A/A and G/G miceThalamic membranes of A/A or G/G mice (mixed gender: 50% of each sex) were pooled and solubilized with 2% Triton X-100. The solubilized preparations were incubated with the wheat germ lectin (WGL) Sepharose 6MB beads and the bound glycoproteins were eluted with 2 × Laemmli sample buffer. The amount of eluate was adjusted to have similar MOPR levels. The eluate was left untreated or treated with PNGase F, resolved with 8% SDS–PAGE, and immunoblotted with anti-mu C (1:5000) as described in Fig.1. This figure is from one of the two independent experiments performed with similar results.
