Immunoblotting of the MOPRs in the thalami and striata of A/A mice, G/G mice and MOPR(-/-) miceBrain membranes were prepared from thalami or striata of adult female mice. Membrane proteins were resolved with 8% SDS–PAGE and immunoblotted with anti-mu C (1:5000), an affinity-purified polyclonal anti-MOR antibody we generated, as described in Materials and Methods. Each figure is a representative of the three independent experiments performed with tissues from different cohorts of female mice. Male mice yielded similar results (not shown).
