A total of 39 SNPs were genotyped in the sample, including the known coding SNP Leu7Pro in NPY (rs16139) located in exon 2. Genotyping was done using a modified single nucleotide extension reaction, with allele detection by mass spectrometry (Sequenom MassArray Sysem; Sequenom, San Diego, CA). All SNP genotypes were checked for Mendelian inheritance using PEDCHECK (O'Connell and Weeks, 1998). Marker allele frequencies and heterozygosities were computed using USERM13 (Boehnke, 1991). Markers were tested for Hardy-Weinberg equilibrium using Haploview (Barrett et al., 2005). No marker deviated significantly (p<0.01) from Hardy-Weinberg equilibrium. Linkage disequilibrium measured by D’ is depicted for NPY, NPY2R, and the NPY1R/NPY5R cluster in Figures 1A, 1B, and 1C.
