Chunk #63 — II. Samples for genome studies of human addiction vulnerabilities and related phenotypes (Figure 2)

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Molecular genetics of addiction and related heritable phenotypes: genome-wide association approaches identify "connectivity constellation" and drug target genes with pleiotropic effects.

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For genotyping, NIMH samples (Sample 8) were divided into seven bipolar and nine control pools of 50–80 subjects/pool. German samples (Sample 9) were divided into 13 bipolar and 10 control pools of 42–60 subjects/ pool. SNP allelic distributions were assessed using duplicate Illumina HumanHap550 assays (Illumina Inc., La Jolla, CA, USA) [2]. Normalized allele frequencies were calculated from raw intensity data averaged across duplicate pools to obtain a relative allele frequency estimate for each SNP in each pool. SNP with allele frequencies that displayed > 2% variance between replicate pools were excluded. t tests compared pool-to-pool variation within phenotypes to phenotype-to-phenotype differences.