Effect of minocycline and naltrexone on ethanol-poly I:C-induced microglial activation and caspase-3 expression: Male C57BL/6 mice were treated with water or ethanol (5 g/kg, i.g.) daily for 10 days. Mice were then injected intraperitoneally with saline, poly I:C (250 μg/kg) and d-GalN (20 mg/kg) in saline 24 hours after the last dose of ethanol. For ethanol-poly I:C-minocycline or naltrexone group, mice were injected with minocycline (50 mg/kg, i.p.) or naltrexone (60 mg/kg, i.p.) 30 minutes before every dose of ethanol or poly I:C treatment. Brain samples were collected three hours after poly I:C and d-GalN administration. All experiments were performed with seven mice per group and repeated two times.
