Chunk #10 — Materials and methods — Real-time PCR analysis

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Chronic ethanol increases systemic TLR3 agonist-induced neuroinflammation and neurodegeneration.

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yes

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Total RNA was extracted from the brain samples of mice treated with ethanol, poly I:C, ethanol-poly I:C or saline, and reverse transcribed as described previously [31]. The primer sequences used in this study were as follows: TNFα, 5′-GAC CCT CAC ACT CAG ATC ATC TTC T-3′ (forward) and 5′-CCT CCA CTT GGT GGT TTG CT-3′ (reverse); IL-1β, 5′-CTG GTG TGT GAC GTT CCC ATT A-3′ (forward) and 5′-CCG ACA GCA CGA GGC TTT-3′ (reverse); IL-6, 5′-GGC CTT CCC TAC TTC ACA AG-3′ (forward) and 5′-ATT TCC ACG ATT TCC CAG AG-3′ (reverse); MCP-1, 5′-ACT GAA GCC AGC TCT CTC TTC CTC-3′ (forward) and 5′-ACT GAA GCC AGC TCT CTC TTC CTC-3′ (reverse); TLR3, 5′-TTG TCT TCT GCA CGA ACC TG-3′ (forward) and 5′-GGC AAC GCA AGG ATT TTA TT-3′ (reverse); HMGB1, 5′-CCA TTG GTG ATG TTG CAA AG-3′ (forward) and 5′-CTT TTT CGC TGC ATC AGG TT-3′ (reverse); gp91phox, 5′-CAG GAG TTC CAA GAT GCC TG-3′ (forward) and 5′-GAT TGG CCT GAG ATT CAT CC-3′ (reverse); β-actin, 5′-GTA TGA CTC CAC TCA CGG CAA A-3′ (forward) and 5′-GGT CTC GCT