NAc punches were homogenized in Trizol (Invitrogen, Carlsbad, CA) and processed according to the manufacturer’s instructions. Purified RNA was reverse transcribed using Bio-Rad iScript Kit (Hercules, CA). cDNA was quantified with real-time PCR using SYBR Green (Applied Biosystems, Foster City, CA). Each reaction was run in triplicate and analyzed using the ΔΔCt method as described previously (Tsankova et al., 2006).
