ChIP was performed for acetylated histone H3 and H4, dimethyl-K9/K27 H3, ΔFosB, and phospho-CREB as described previously (Kumar et al., 2005) with minor modifications. Immunoprecipitated DNA was amplified via ligation-mediated PCR and hybridized to NimbleGen mouse MM8 promoter arrays. See Supplemental Information for detailed ChIP-chip methods. Quantitative ChIP was performed as described above except that the immunoprecitated DNA was directly quantified by quantitative real-time PCR.
