mouse was placed in a cylindrical container in one side chamber, while an empty cylindrical container was placed in the opposite side chamber. The mouse was then recorded for 10 min, and scored with respect to the amount of time it spent with the stranger mouse vs. the empty compartment. 3) Novel Object Recognition Each test mouse was placed in an empty 1 ft2 testing chamber for 5 min to acclimate, then removed, and two identical objects were placed in the chamber. The mouse was returned to the chamber with the objects, placed facing directly away from them, recorded for 10 min and scored for time spent in proximity to each object (Bevins and Besheer, 2006). After one hour, the experiment was repeated, with one of the two objects replaced by a novel object. 4) Pre-pulse inhibition Each mouse was placed in a restraint chamber inside a larger isolation cabinet, equipped with sound, light, and air puff generators (SR-LAB, San Diego Instruments), and auditory PPI assessed as described (Geyer and Swerdlow, 2001). 5) Sucrose preference This experiment was always performed last, as mice were individually housed in order to measure liquid consumption. Sucrose preference was determined by the percentage of
