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Chunk #43 — STAR METHODS — METHOD DETAILS — Behavior

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Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia.
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Tests were performed in the same sequence for all mice, and included: 1) Elevated Plus Maze Each test mouse was placed in the center of a raised, plus-shaped apparatus, consisting of 2 enclosed arms and 2 open arms, facing an open arm (Walf and Frye, 2007). Each tested mouse was videotaped and scored for time spent in the open vs. closed arms. 2) Three chamber social choice The test apparatus is a plexiglass enclosure divided into thirds with connecting doors (Ugo Basile, Italy) (Yang et al., 2011). Each test mouse was first acclimated to the central chamber or 5 min. The doors to the outer chambers were then removed, and the test mouse allowed to explore all three chambers for 10 min. The test mouse was then guided back to the central chamber, and a same sex and age stranger mouse was placed in a cylindrical container in one side chamber, while an empty cylindrical container was placed in the opposite side chamber. The mouse was then recorded for 10 min, and scored with respect to the amount of time