To determine if blood-forming potential differs among cell lines that satisfy more stringent criteria for pluripotency, we analyzed lines derived from a uniform genetic background (B6/129F1) that all express a Nanog-eGFP reporter gene28, and for which pluripotency was demonstrated by blastocyst chimerism and transmission through the germ line (Fig. 4a, upper schema; Supplementary Table 6). These studies involve “secondary” iPSC lines derived from neural progenitor cells (NP-iPSC29) and B-lymphocytes (Bl-iPSC30) of mice chimerized with iPSC carrying proviruses that express doxycycline-inducible reprogramming factors from identical proviral integration sites. NP-iPSC and Bl-iPSC were compared to ntESC generated from neural progenitor cells (NP-ntESC8), blood progenitor cells (B-ntESC31), and fibroblasts (F-ntESC31, 32), as well as fESC.
