All cell lines were differentiated into embryoid bodies and assayed for hematopoietic colony forming activity16. Across multiple clones, we observed higher blood forming potential of iPSC derived from B lymphocytes (Bl-iPSC) than from neural progenitors (NP-iPSC; Fig. 4b). In contrast, we observed that ntESC, regardless of tissue origin (fibroblasts, neural progenitors, or T-cells), and fESC displayed an equivalently robust blood forming potential (Fig. 4b). In this independent set of iPSC lines, qualified as pluripotent by stringent criteria, we again observed consistent differences in blood formation, with blood derivatives showing more robust hematopoiesis in vitro than neural derivatives.
